After Biden announced his withdrawal from the election, Republican House Speaker Johnson called on Biden to resign "immediately". Johnson said in a statement, "If Biden is not suitable to run for president, he is not suitable to be president. He must immediately resign from the presidency." Johnson said that more than 14 million Americans chose Biden as the Democratic presidential candidate, but the party that called itself "Democratic" announced that their votes were invalid. As the Speaker of the House of Representatives, Johnson ranks second in the presidential succession order, second only to Harris.

When asked about some Republican congressmen calling on Biden to resign as president, the White House said Biden "looks forward to completing his term." Senate Republican leader McConnell also accused the Democrats of "trying to overturn the will of the American people expressed in the national primaries" in a statement, but did not call for Biden to resign as president.